RAPD Markers are Associated with Self-incompatibility Characteristics as Related to the Number of Seeds per Fruit of Some Mandarin and Clementine Cultivars

Document Type : Original Article


1 Vegetable Breeding, Horticulture Inst., ARC, Giza, Egypt.

2 Hort. Res. Inst., Agric. Res. Centre, Egypt

3 Horticulture Research Institute, ARC, Giza 12619, Egypt

4 Botany Department, Women’s College for Arts, Science and Education, Ain Shams University, Cairo 11566, Egypt

5 Department of Horticulture, Faculty of Agriculture, Ain Shams University, Cairo 11241, Egypt

6 Pomology Department, Faculty of Agriculture, Assiut University, Assiut, Egypt

7 Horticultural Department, Faculty of Agriculture, Suez Canal University, 41522, Egypt


The experiments were performed using (Nour, Fedela, Spinosa clementines, Kishu seedless, Balady, and Seedless mandarins) to determine the compatibility or incompatibility as related to the number of seeds per fruit and the genetic relation between them. For two consecutive seasons (2020 and 2021), the growth of the pollen tube in the styles following self-pollination has been analyzed using a fluorescence microscope to detect incompatibility properties. According to Nour and Fedela clementine cultivars are highly incompatible, while the other four cultivars are incompatible. After receiving pollen, Nour and Fedela clementines had very low pollen tubes that reached style. On the other hand, the highest rate was in the other four cultivars. The DNA of the six cultivars of mandarin and clementine investigated in this study was very similar. Balady mandarin and seedless mandarin appear to have very high similarities, while Fedela clementine and seedless mandarin appear to be very low. RAPD-PCR showed a specific molecule related to incompatibility such as (A-18) RAPD primer, at MW 361 bp. linked to the incompatibility of Nour and Fedela clementine c.vs.