Using Different Methods to Produce Vegetative Carob Seedlings

A FIELD experiment was conducted during 2014-2015 to study the influence of different indole butyric acid (IBA) concentration (4000, 6000 and 8000 ppm) on rooting of Carob air layers and cuttings at Horticulture Research Institute Egypt, during February, May, August and November. The results indicated that, May is the best time to make air layering. Applying IBA at 4000 ppm had recorded the longest root for genotypes (1, 3 and 4) in both seasons. IBA at 6000 ppm gave the best percentage of rooting and survival and number of root for genotype (1) and longest root for genotype (5) while, treated with IBA at 8000 ppm registered the highest percentage of rooting and survival also the number of root for genotypes (3- 4 and 5). The percentage of rooting and survival Carob stem cuttings was very low (35 and 51.7% maximum) respectively. The genetic polymorphism between the four Carob genotypes was detected by RAPD analysis. Twenty eight out of 53 bands detected were polymorphic for the different genotypes. Six RAPD specific markers distinguish genotype 5 followed by genotype 4 (four markers) and both genotypes 1 and 3 showed only two specific markers.