Molecular and Morphological Variations Induced by Gamma Rays in Yardlong Bean

Ezzat, Asmaa Salah; Ahmed, Hassan Soltan; Osman, Sayed; Gad El-Hak, Seif El-Nasr Hussein

doi:10.21608/ejoh.2020.53855.1157

Molecular and Morphological Variations Induced by Gamma Rays in Yardlong Bean

Document Type : Original Article

Authors

¹ Horticulture department, Faculty of Agriculture, Minia university

² Agriculture Research Center

³ Genetics Department, Faculty of agriculture, Minia university

⁴ Departmen of vegetable - Faculty of Agriculture- El-Minia Univerisity Egypt

10.21608/ejoh.2020.53855.1157

Abstract

This study was carried out to identify the genetic variations in yardlong bean plants irradiated by various doses (75, 150, 300, 450 and 600Gy) of gamma rays using RAPD and ISSR markers techniques as well as, their effect on some vegetative traits were studied. ten RAPD and 10 ISSR markers were used in this investigation. The obtained results showed that the ten RAPD primers produced 117 bands only 94 of them were polymorphic while 23 bands were monomorphic. The percentages of polymorphism among primers ranged from 0.0% to 100%. All of the RAPD primers produced unique bands except OPK-06 primer which did not produced any unique bands. Meanwhile, 62 amplified bands including 26 polymorphic and 36 monomorphic bands were generated by ten ISSR primers. All of the ISSR primers did not produce any unique bands except ISSR4 primer which produced only one unique band, these results demonstrated that this unique sequence could be used as a molecular marker associated with gamma irradiation. The field results showed that the emergence percentage decreased with increasing doses up to 600Gy treatment which gave the lowest emergence percentage. Inconstant values of vegetative characteristics were obtained after each treatment with gamma ray doses; 300Gy treatment gave the highest value of plant height, number of branches, pod length and number of seeds per pod compared to control and other treatments. The values of coefficients of phenotypic and genotypic variation, heritability and expect genetic advance did not follow regular increase or decrease with the radiation doses in both seasons. The present data demonstrate that using gamma rays to induce DNA polymorphism in this plant genome which might be lead to appearance of a desired phenotype and genetic characteristics which could be used in yardlong bean improvement programs.

Keywords