Relationship Between the Use of Gallic Acid and Genetic Stability of Grapes

Elbotaty, Elshaima M; Heiba, Samy; Saleh, Mohamed Maher Saad

doi:10.21608/ejoh.2023.166052.1214

Relationship Between the Use of Gallic Acid and Genetic Stability of Grapes

Document Type : Original Article

Authors

¹ Viticulture Research Department, Horticulture Research Institute, Agricultural Research Centre, Giza, Egypt.

² Genetics and Cytology Department, National Research Centre, Giza, Egypt.

³ Pomology Department, National Research Centre, Giza, Egypt

10.21608/ejoh.2023.166052.1214

Abstract

The effect of Gallic acid (GA) at 200, 300, and 350 mg L-1, on the genetic stability of SO4 and Freedom micropropagated grape rootstocks was studied through two subcultures. Plantlets’ vegetative parameters didn’t differ significantly between both primary subcultures. An inverse correlation occurred between elevated GA concentration in culture medium and plantlets’ vegetative growth, meanwhile, it was a promoter by a moderate concentration of 200 mg L-1. Furthermore, when GA exceeded 350 mg L-1 caused plantlets’ breakdown. Moreover, fingerprinting analysis, RAPD-PCR has been performed to investigate relationships among GA presences in culture media during subcultures and their genetic profile. It detected 93 monomorphic bands with a ratio of 73.8 % and 18 polymorphic bands with a ratio of 16.2 %, out of them, 11 in the 1st subculture, while 7 in the 2nd subculture were detected. Under GA concentrations with a total ratio of 16.2 %, the polymorphism ratio was 61.1 % in the 1st subculture and decreased to 38.9 % in the 2nd subculture using 350 mg L-1 of GA. Gallic acid concentrations, of 200, 300 and 350 mg L-1 in growth medium preserved both micro-propagated rootstocks’ genetic stability through two subcultures.

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