Horticulture Evaluation and Genetic Identification of Selected Le-Conte Pear Clone

Document Type : Original Article

Abstract

 ASELECTED clone of Le- Conte pear was evaluated in ……comparison to Le-Conte old cultivar. Flowering of the selected clone was earlier by 7- 10 days. Maturity was 25-30 days earlier than the old cultivar. Whereas it produced fruits fewer than the old cultivar Le-Conte under the same conditions. No significant differences were observed between the new clone and the old cultivar within the vegetative growth . However, fruit physical prosperities increased significantly in the selected clone compared to the old cultivar. Chemically, the selected clone revealed a higher ratio of T.S.S / acidity than in Le-Conte cultivar. The same trend was noticed within total and reducing sugars. Yield was higher in the old cultivar than in the selected clone.
Screening of DNA by RAPD marker showed a total number of 74 amplicons with an average of 7-4 amplicon/ primer when ten primers were used. The highest number of polymorphic amplicon (2) produced by OPA17.The size of fragment varied from 280-1790 bp. Two negative unique markers were detected with the selected clone by OPA 17. These markers were located at 550 bp and150 bp. Meanwhile, OPG 06 revealed an unique positive marker at 450 bp.The estimated similarity between the selected clone and the old cultivar was 95.3.